Spectral multiplex imaging

Spectral immunofluorescent imaging allows you to combine up to 15 markers, stained and imaged at the same time, through a combination of fluorophores with similar excitation wavelengths but distinct emission wavelengths. We use this multiplex imaging to investigate the heterogeneity of macrophages in their tissue micro-environment. Several possible applications have been described in Goossens et al., Cell Metabolism 2022, while a step-by-step protocol can be found in Wieland et al., STAR Protocols 2023 (see below).

Try it yourself: Combine different markers in this 9-plex stained image of a murine atherosclerotic plaque:

Some examples of multiplex spectral immunofluorescent stainings in tissue sections from different organs: